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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be observed in several tumors, which ends up in chromosome instability, and thus may add to tumorigenesis. This protein was located to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a crucial role in endorsing cell-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already described. Two more splice variants had been claimed but specific nucleotide sequence details is not nevertheless accessible.
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Blocking peptides are peptides that bind specifically to the focus on antibody and block antibody binding. These peptide ordinarily includes the epitope identified by the antibody.
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This guideline outlines even more achievable optimization of cellular and immunoassay parameters to be certain the very best final results are...
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This guideline outlines even further possible optimization of cellular and immunoassay parameters to make sure the best possible final results are...
Antibodies certain to the blocking peptide now not bind towards the epitope within the focus on protein. By comparing the staining through the blocked antibody compared to the antibody by yourself, you can see which staining is precise.
Focus on Details Cyclin E belongs for the very conserved cyclin family members, whose members are characterised by a remarkable periodicity in protein abundance with the mobile cycle. Cyclins function as regulators of CDK kinases. Unique cyclins exhibit distinctive expression and degradation patterns which add on the temporal coordination of every mitotic occasion. Cyclin E sorts a fancy with and functions being a regulatory subunit of CDK2, whose activity is needed for mobile cycle G1/S transition. This protein accumulates for the G1-S phase boundary which is degraded as cells progress by way of S section.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both equally antibodies...
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